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AssigneeHenkel AG & Co. KGaA
InventorsCornelius BESSLER, Susanne WIELAND, Karl-Heinz MAURER
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The present invention relates to α-amylase variants that are stabilized to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, by point mutagenesis of asparagine (N) or glutamine (Q) residues located on the surface of the molecule to give other amino acid residues. The invention further relates to methods of increasing the stability of an α-amylase to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, whereby at least one asparagine (N) or glutamine (Q) residue on the surface of the molecule is replaced with a different amino acid residue. The α-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.
An α-amylase variant having at least one amino acid substitution with respect to a predetermined α-amylase starting molecule, wherein in said at least one amino acid substitution an asparagine (N) or a glutamine (Q) residue on the surface of said starting molecule is replaced with A, C, F, G, H, I, K, L, M, P, R, S, T, V, W or Y in the variant.
An α-amylase variant according to 1, wherein the starting molecule is selected from the group consisting of α-amylase from Bacillus sp. A 7-7 (DSM 12368), α-amylase from Bacillus sp. #707, a amylase from Bacillus sp. KSM-AP1378, α-amylase from Bacillus KSM-K38, α-amylase from B. amyloliquefaciens, α-amylase from B. licheniformis, α-amylase from Bacillus sp. MK716, α-amylase from Bacillus sp. TS-23, α-amylase from B. stearothermophilus, α-amylase from B. agaradherens, and cyclodextrin glucanotransferase (CGTase) from B. agaradherens;
A method of preparing a variant α-amylase having increased stability with respect to solvent-exerted hydrolysis in comparison with a predetermined α-amylase starting molecule, the method comprising:
Dependencies are inferred from the claim text.