Process for purification and recovery of paclitaxel compounds

Abstract

A method for producing paclitaxel from a broth of cell cultures includes the steps of (a) providing an aqueous broth of cell cultures including cells having intracellularly associated paclitaxel; (b) contacting the broth with a hydrophobic resin; (c) adsorbing the intracellularly associated paclitaxel onto the resin; and (d) separating the paclitaxel from the resin. The contacting of the broth with the resin may include the step of forming an admixture of the broth and the resin. The amount of the resin in the admixture may be from about 1 to about 10 grams of resin per 100 mL of broth, more desirably, from about 2 to about 6 grams of resin per 100 mL of broth. Useful cell cultures includes cell cultures of Taxus species, such as T. baccata, T. brevifolia, T. canadensis, T. chinensis, T. cuspidata, T. floridana, T. globosa, T. media, T. wallichiana and combinations thereof. Useful hydrophobic resins include brominated styrenic polymeric resins.

Dependencies are inferred from the claim text.