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US 2007/0190621 A1Published application
Synthesis of oseltamivir carboxylates
AssigneeBoard of Trustees of Michigan State University
InventorsJohn Frost, Jiantao Guo
Life of this patent
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Abstract
Enzymatic pathways for production of aminoshikimate, kanosamine, intermediates, and derivatives thereof; nucleic acid encoding and cells containing the enzymes; compositions containing aminoshikimate, kanosamine, an intermediate or derivative thereof; and use of the cells and pathways for biosynthetic production of aminoshikimate, kanosamine, intermediates, and derivatives thereof.
Claims76
An isolated or recombinant aminoshikimate biosynthesis enzyme system comprising:
- (1) a 3-keto-D-glucose-6-phosphate (3KG6P) dehydrogenase,
- (2) a 3-keto-D-glucose-6-phosphate (3KG6P) transaminase, and
- (3) a 4-amino-3,4-dideoxy-D-arabino-heptulosonic acid 7-phosphate (aminoDAHP) synthase,
- wherein the enzyme system is capable of catalyzing conversion of glucose-6-phosphate (G6P) to 3-keto-D-glucose-6-phosphate (3KG6P), 3KG6P to kanosamine-6-phosphate (K6P), K6P to 1-imino-1-deoxy-D-erythrose-4-phosphate (iminoE4P), iminoE4P to 4-amino-3,4-dideoxy-D-arabino-heptulosonic acid 7-phosphate (aminoDAHP), and aminoDAHP to aminoshikimate.
1 more element
An isolated or recombinant kanosamine biosynthesis enzyme system comprising:
- (1) at least one 3-keto-D-glucose-6-phosphate (3KG6P) dehydrogenase,
- (2) at least one 3-keto-D-glucose-6-phosphate (3KG6P) transaminase, and
- (3) at least one K6P phosphatase,
- wherein the enzyme system is capable of catalyzing the conversion of glucose-6-phosphate (G6P) to 3-keto-D-glucose-6-phosphate (3KG6P), 3KG6P to kanosamine-6-phosphate (K6P), and K6P to kanosamine.
1 more element
Dependencies are inferred from the claim text.