Novel essential fungal polynucleotides, polypeptides, and methods of use
AssigneeBristol-Myers Squibb Company
InventorsYing-Kai Wang, Mengping Liu, Brian Dougherty, Matthew Healy, Daniel Davison, Charles Mazzucco, Stanley Krystek, Donna Bassolino, Trina Maurice
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Abstract
The present invention provides essential fungal polynucleotides and their encoded polypeptides, homologues thereof and their uses. Additionally, the invention provides methods for the identification of essential polynucleotides and fungal strains which may be used for drug screening.
Claims13
1-30. (canceled)
An isolated polypeptide comprising a sequence selected from the group consisting of:
- (a) an isolated polypeptide comprising amino acids 1 to 346 of SEQ ID NO:12;
- and
- (b) an isolated polypeptide comprising amino acids 2 to 346 of SEQ ID NO:
- 12;
- wherein said encoded polypeptide has keto-steroid reductase activity.
An isolated polypeptide encoded by a polynucleotide consisting of nucleotides 1 to 1038 of SEQ ID NO:
- 1.
An isolated polypeptide encoded by a polynucleotide consisting of nucleotides 4 to 1038 of SEQ ID NO:
- 1.
An isolated polypeptide comprising a polypeptide that is at least 95.0% identical to amino acids 2 to 346 of SEQ ID NO:
- 12, wherein percent identity is calculated using a CLUSTALW global sequence alignment using default parameters, and wherein said polypeptide has keto-steroid reductase activity.
An isolated polypeptide comprises at least 318 contiguous amino acids of SEQ ID NO:
- 12, wherein said polypeptide has keto-steroid reductase activity.
An isolated polypeptide comprising amino acids 29 to 346 of SEQ ID NO:12.
An isolated polypeptide comprising amino acids 1 to 318 of SEQ ID NO:
- 12.
Dependencies are inferred from the claim text.