Modified integrase and methods of use
AssigneeAviGenics, Inc.
InventorAlex Harvey
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Abstract
The invention includes modified enzymes such as integrases which maintain all or part of their enzymatic activity and can localize to the nucleus.
Claims33
An enzyme which facilitates insertion of a nucleotide sequence into the genome of a cell wherein the enzyme has been modified to contain an NLS.
A serine integrase containing an NLS.
A phiC31 integrase containing an NLS.
A mutant integrase wherein an NLS is contained in the integrase amino acid sequence at a position that is nonconserved.
A phiC31 integrase wherein the NLS is inserted between amino acid 228 and amino acid 242.
A cell containing an integrase which contains an NLS wherein the cell contains a nucleotide sequence comprising a heterologous coding sequence.
A method comprising introducing an integrase-NLS fusion protein and nucleic acid which comprises an integration sequence into a cell wherein the cell contains an integration site in its genome.
Dependencies are inferred from the claim text.