Oligosaccharide modification and labeling of proteins
AssigneeInvitrogen Corporation
InventorsBrian AGNEW, Schuyler Corry, Kyle Gee
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Abstract
The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
Claims22
A method of producing a glycomodified protein, comprising
- attaching a modified sugar comprising a chemical handle to a GlcNAc residue on a first protein;
- and
- mixing said first protein with a reporter molecule, carrier molecule or solid support capable of reacting with said chemical handle;
- wherein said reporter molecule, carrier molecule or solid support attaches to the protein at said chemical handle, thereby forming a glycomodified protein.
A method of labeling an protein by labeling an oligosaccharide attached to said protein, comprising incubating an protein-producing cell in the presence of an unnatural sugar, wherein said unnatural sugar comprises a chemical handle.
An antibody comprising a labeled oligosaccharide.
Dependencies are inferred from the claim text.