Density enhanced protein tyrosine phosphatases
AssigneeCold Spring Harbor Laboratory
InventorsNicholas Tonks, Arne Ostman
Life of this patent
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Abstract
Novel Type III density enhanced protein tyrosine phosphatases are disclosed and exemplified by human DEP-1 enzyme. Polynucleotides encoding huDEP-1 are disclosed, along with methods and materials for production of the same by recombinant procedures. Binding molecules specific for DEP-1 are also disclosed as useful for modulating the biological activities of DEP-1.
Claims14
(canceled)
An immunogen selected from:
- (a) a polypeptide that comprises the amino acid sequence set forth in SEQ ID NO:2;
- (b) a polypeptide that comprises a human Type III density enhanced protein-1 tyrosine phosphatase polypeptide that is encoded by a DNA molecule that hybridizes at 65° C. in a buffer containing 40 mM Na2HPO4, 1% SDS, and 1 mM EDTA to the complement of the protein coding portions of the DNA sequence set forth in SEQ ID NO:
- 1;
- (c) a polypeptide consisting of the extracellular region polypeptide of 940 consecutive amino acids of SEQ ID NO:2, said extracellular region polypeptide comprising an amino acid sequence with an amino terminus of Gly located at residue 37 of SEQ ID NO:2;
- and
- (d) a huDEP-1 polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, wherein said huDEP-1 polypeptide is present on the extracellular membrane surface of a cell;
- (e) a polypeptide that comprises an intracellular portion of 341 amino acids of a human Type III density enhanced protein-1 tyrosine phosphatase polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, wherein the intracellular portion comprises amino acids residues 1060-1296 of SEQ ID NO:2, and wherein the intracellular portion does not include amino acids 977-996 of SEQ ID NO:2;
- and
- (f) a polypeptide that comprises an amino acid sequence as set forth at positions 997-1337 of SEQ ID NO:2;
- (g) a polypeptide fragment comprising an amino acid sequence as set forth at positions 977-996 of SEQ ID NO:2;
- (h) a polypeptide fragment comprising an amino acid sequence as set forth at positions 1060-1296 of SEQ ID NO:2
- (i) a polypeptide fragment comprising an amino acid sequence as set forth at positions 1297-1315 of SEQ ID NO:2;
- and
- (j) a polypeptide fragment comprising an amino acid sequence as set forth at positions 1321-1334 of SEQ ID NO:2.
3-11. (canceled)
13-15. (canceled)
An antibody that specifically binds to a human Type III density enhanced protein-1 tyrosine phosphatase polypeptide that is encoded by a DNA molecule that hybridizes at 65° C. in a buffer containing 40 mM Na2HPO4, 1% SDS, and 1 mM EDTA to the complement of the protein coding portions of the DNA sequence set forth in SEQ ID NO:1.
An antibody that specifically binds to a polypeptide fragment:
- (a) a polypeptide fragment comprising an intracellular portion of 341 amino acids of a human Type III density enhanced protein-1 tyrosine phosphatase polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, wherein the intracellular portion comprises amino acids residues 1060-1296 of SEQ ID NO:2, and wherein the intracellular portion does not include amino acids 977-996 of SEQ ID NO:2;
- (b) a polypeptide fragment comprising the amino acid sequence as set forth at positions 997-1337 of SEQ ID NO:2;
- (c) a polypeptide fragment comprising the amino acid sequence as set forth at positions 977-996 of SEQ ID NO:2;
- (d) a polypeptide fragment comprising the amino acid sequence as set forth at positions 1060-1296 of SEQ ID NO:2;
- (e) a polypeptide fragment comprising the amino acid sequence as set forth at positions 1297-1315 of SEQ ID NO:2;
- and
- (f) a polypeptide fragment comprising the amino acid sequence as set forth at positions 1321-1334 of SEQ ID NO:2.
19-24. (canceled)
29-42. (canceled)
Dependencies are inferred from the claim text.