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US 2007/0190563 A1Published application
Primer and primer set for amplification of CEA nucleic acid, and method for assisting cancer diagnosis
AssigneeSysmex Corporation
InventorMotonari Daitho
Life of this patent
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Abstract
The prdsent invention provides a nucleic acid amplification primer which can detect CEAmRNA with sufficient reproducibility than the Conventional nualeic acid amplification primer for carcinoeibryonic antigen: CEA detection, nucleic acid amplification primer sets, and methods
Claims20
A nucleic acid amplification primer for detecting mRNA of carcinoembryonic antigen (CEA) comprising:
- a first base sequence being capable of hybridizing to a first target region or a second target region wherein the first base sequence is located at the 3′ end of primer and the first target region consists of the 704th to 1237th base sequences of SEQ ID NO:
- 1, and the second target region consists of a complementary base sequence for the first target region;
- and
- a second base sequence being capable of hybridizing to an elongated strand from the first base sequence wherein the second base sequence is located at the 5′ end of primer.
1 more element
A nucleic acid amplification primer set for detecting mRNA of carcinoembryonic antigen (CEA) comprising:
- a first primer containing a first base sequence being capable of hybridizing to a first target region at the 3′ end and a second base sequence being capable of hybridizing to an elongated strand from the first base sequence at the 5′ end and the first target region consists of the 704th to 1237th base sequences of SEQ ID NO:
- 1;
- a second primer containing a third base sequence being capable of hybridizing to a region further downstream from the region in which the second base sequence is capable of hybridizing in the elongated strand from the first base sequence at the 3′ end and a fourth base sequence being capable of hybridizing to an elongated strand from the third base sequence at the 5′ end;
- and
- a third primer being capable of hybridizing to a region further downstream from the region in which the third base sequence is capable of hybridizing in the elongated strand from the first base sequence.
2 more elements
A nucleic acid amplification primer set for detecting mRNA of carcinoembryonic antigen (CEA) comprising:
- a first primer containing a first base sequence being capable of hybridizing to a first target region at the 3′ end and a second base sequence being capable of hybridizing to an elongated strand from the first base sequence at the 5′ end and the first target region consists of the 704th to 1237th base sequences of SEQ ID NO:
- 1;
- a second primer containing a third base sequence being capable of hybridizing to a region further downstream from the region in which the second base sequence is capable of hybridizing in the elongated strand from the first base sequence at the 3′ end and a fourth base sequence being capable of hybridizing to an elongated strand from the third base sequence at the 5′ end;
- and
- a third primer being capable of hybridizing to a region further downstream from the region in which the third base sequence is capable of hybridizing in the elongated strand from the first base sequence.
- a fourth primer being capable of hybridizing to a region between the 3′ end of the first base sequence and the 5′ end of a sequence in which the second base sequence is capable of hybridizing in the elongated strand from the first base sequence;
- and
- a fifth primer being capable of hybridizing to a region between the 3′ end of the third base sequence and the 5′ end of a sequence in which the fourth base sequence is capable of hybridizing in the elongated strand from the the third base sequence.
5 more elements
Dependencies are inferred from the claim text.