Isolation of nucleic acid
AssigneeInvitrogen Dynal AS
InventorsArne Deggerdal, Frank Larsen
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Abstract
The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.
Claims20
A method of isolating genomic DNA from a sample comprising cells, wherein the genomic DNA is present in the cells, the method comprising (a) contacting the sample with a solid support comprising an organic polymer, wherein the solid support selectively binds the cells, (b) contacting the cells bound to the solid support with a detergent in the absence of any chaotropic agent, whereby soluble genomic DNA becomes bound to the support in a sequence-independent manner in the presence of the detergent and absence of any chaotropic agent, and (c) separating the support with bound genomic DNA from the sample.
A method of isolating RNA and genomic DNA from a sample comprising cells, wherein the RNA and genomic DNA are present inside the cells, the method comprising (a) contacting the sample with a solid support comprising an organic polymer, wherein the solid support selectively binds the cells, (b) contacting the cells bound to the support with a detergent in the absence of any chaotropic agent, whereby soluble genomic DNA in the sample is bound to the support in a sequence-independent manner in the presence of the detergent and absence of any chaotropic agent;
- (c) separating the support with bound genomic DNA from the sample;
- and (d) isolating RNA from the sample.
Dependencies are inferred from the claim text.