Multiplex PCR assay
OwnerVishali Gupta, Naresh Sachdeva +3inventors
InventorsVishali Gupta, Naresh Sachdeva, Amod Gupta, Sunil Arora, P. Bambery
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Abstract
This invention relates to a multiplex PCR assay capable of screening or detecting the relevant microbial organism specific to Mycobacterium tuberculosis, Toxoplasma gondii in a sample, comprising a reaction mixture of a combination of 3 sets of primers, one pair of said primers for detection of Mycobacterium tuberculosis, a second pair of said primers for detection of Toxoplasma gondii and a third pair of primers for the detection of pathogenically important fungi, said primers being compatible to each other.
Claims7
A multiplex PCR assay capable of screening or detecting the relevant microbial organism specific to Mycobacterium tuberculosis, Toxoplasma gondii in a sample comprising a reaction mixture of a combination of 3 sets of primers, one of said primers for detection of Mycobacterium tuberculosis, a second of said primers for detection of Toxoplasma gondii and a third primer pair for the detection of pathogenically important fungi, said primers being compatible to each other.
A method for identifying the relevant microbial organism in a sample comprising the steps of:
- preparing a mixture of primers compatible to each other;
- treating the clinical sample with the said primers after initial denaturation thermo cycling being carried our for 35 cycles with denaturation, annealing at 54° C. for 45 sec and extension at 72° C. also for 45 sec with last cycle of extension of 5 mins
- detecting the relevant micro-organism after amplification of the specific gene targets on 25% agarose gel.
Dependencies are inferred from the claim text.