Hybridization assisted nanopore sequencing

A method for determining the sequence of a biomolecule strand of interest, comprising the steps of:

• providing a sequencing apparatus having a first fluid chamber, a second fluid chamber, a membrane positioned between said first and second chambers and a nanopore extending through said membrane such that said first and second chambers are in fluid communication via said nanopore;
• providing a single-stranded biomolecule;
• providing a first plurality of matching probes having a known sequence;
• hybridizing said first plurality of probes with said single-stranded biomolecule such that said first plurality of probes attach to portions of said single-stranded biomolecule to produce a partially hybridized biomolecule;
• introducing said partially hybridized biomolecule into said first chamber;
• translocating said partially hybridized biomolecule from said first chamber through said nanopore and into said second chamber;
• monitoring changes in current across said nanopore as said partially hybridized biomolecule is translocated therethrough, said changes in electrical potential corresponding to locations along said partially hybridized biomolecule containing one of said first plurality of probes;
• and
• recording said changes in electrical potential as a function of time.