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US 2007/0190535 A1Published application
Size fractionation of nucleic acid samples
OwnerGerald Hall, Michael Barrettinventors
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Abstract
Methods for size fractionating nucleic acid molecules, using glass fiber filtration columns are described. The methods are useful in many downstream applications including array-based comparative genomic hybridization (aCGH), PCR-based sequencing, somatic genotypic with polymorphic markers, etc.
Claims18
A method for fractionating nucleic acid molecules according to size, comprising:
- preparing a buffered nucleic acid composition by combining a sample of nucleic acids with a solution of binding buffer;
- contacting the buffered nucleic acid composition to a glass fiber filtration medium;
- and
- washing the glass fiber filtration medium with a wash medium to fractionate the nucleic acid sample according to size,
- the binding buffer including a chaotropic salt;
- the wash medium comprising a lower alcohol, and the concentration of the chaotropic salt and lower alcohol being selected to separate a nucleic acid of a desired size.
3 more elements
A method for enriching a sample of genomic DNA with DNA fragments of a desired size, comprising:
- preparing a DNA sample containing one or more DNA fragments of different sizes in a solution of binding buffer containing a chaotropic salt;
- contacting the DNA sample to a glass fiber filtration medium;
- washing the glass fiber filtration medium with alcohol;
- and
- eluting the DNA fragments bound to the separation medium,
- the concentrations of chaotropic salt and alcohol being selected to separate DNA fragments of a desired size.
3 more elements
A kit for size separation of a nucleic acid sample, comprising:
- at least one glass fiber filtration column;
- a binding buffer used to apply a nucleic acid sample to the glass fiber filtration column;
- and
- a wash solution for removing the nucleic acid sample from the glass fiber filtration column.
1 more element
Dependencies are inferred from the claim text.