Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same

A method of determining the presence or absence of a mutation in a nucleic acid sequence contained in a nucleic acid sample, the method comprising:

• (a) preparing a nucleic acid sample;
• (b) preparing a primer set that allows a target nucleic acid sequence containing a site associated with a mutation to be amplified, the primer set being designed so that when at least one primer included in the primer set hybridizes to a nucleic acid sequence contained in the nucleic acid sample or a complementary sequence thereto, at least one mismatch occurs between the at least one primer and the nucleic acid sequence or the complementary sequence thereto, depending on the presence or absence of the mutation;
• and
• (c) performing a nucleic acid amplification reaction in the presence of a substance having mismatch recognition ability, using the primer set in which the nucleic acid sample serves as a template.

A method of determining the presence or absence of a mutation in a nucleic acid sequence contained in a nucleic acid sample, the method comprising:

• (a) preparing a nucleic acid sample;
• (b) preparing a primer set that allows a target nucleic acid sequence containing a site associated with a mutation to be amplified;
• (c) preparing a nucleic acid fragment that hybridizes to the target nucleic acid sequence and that is designed so that when the nucleic acid fragment hybridizes to a nucleic acid sequence contained in the nucleic acid sample or a complementary sequence thereto, at least one mismatch occurs between the nucleic acid fragment and the nucleic acid sequence or the complementary sequence thereto, depending on the presence or absence of the mutation;
• and
• (d) performing a nucleic acid amplification reaction in the presence of a substance having mismatch recognition ability and the nucleic acid fragment, using the primer set in which the nucleic acid sample serves as a template.

A kit for determining the presence or absence of a mutation in a nucleic acid sequence contained in a nucleic acid sample, the kit comprising:

• (a) a substance having mismatch recognition ability;
• and
• (b) a primer set that allows a target nucleic acid sequence containing a site associated with a mutation to be amplified;
• and
• (c) a nucleic acid fragment that hybridizes to the target nucleic acid sequence, the nucleic acid fragment being designed so that when the nucleic acid fragment hybridizes to the nucleic acid sequence contained in the nucleic acid sample or a complementary sequence thereto, at least one mismatch occurs between the nucleic acid fragment and the nucleic acid sequence or the complementary sequence thereto, depending on the presence or absence of the mutation.