Method for bisulfite treatment
AssigneeRoche Molecular Systems, Inc.
InventorsChristian Birkner, Christine Markert-Hahn, Herbert von der Eltz
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Abstract
The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.
Claims11
Method for the conversion of a cytosine base, in a nucleic acid to an uracil base comprising:
- a) providing a solution that contains a nucleic acid,
- b) providing guanidinium hydrogen sulfite and preparing a solution comprising guanidinum and sulfite ions,
- c) mixing the solutions from step a) and b),
- d) incubating the solution obtained in step c) containing the nucleic acid and guanidinium and sulfite ions whereby the nucleic acid is deaminated,
- e) incubating the deaminated nucleic acid under alkaline conditions whereby the deaminated nucleic acid is desulfonated, and
- f) isolating the deaminated nucleic acid.
9-12. (canceled)
A kit containing guanidinium hydrogen sulfite and plasticware for performing a reaction in which a cytosine base in a nucleic acid is converted to a uracil base.
(canceled)
Dependencies are inferred from the claim text.