Tissue-specific basement membrane gels
OwnerEric Brey, Shiri Uriel +2inventors
InventorsEric Brey, Shiri Uriel, Edwardine Labay, Ralph Weichselbaum
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Abstract
Methods are provided for basement membrane extraction and gellation whereby a biologically active, stable, three dimensional gel can be induced within five minutes or less with basement membrane components from substantially any tissue selected by the preparer. The three dimensional gel can further be substantially free of cellular contaminants. Thus, a wide range of diagnostic and therapeutic uses for such gels can be made available.
Claims24
A method of obtaining a gel structure including a protein extract from a tissue, the method comprising:
- selecting a tissue for extraction of basement-membrane proteins;
- obtaining a solution of the basement membrane components from the tissue;
- gelling the extracted basement membrane by at least one of lowering the pH of the solution of the extracted basement membrane components or adding cells to the solution of the extracted basement membrane.
A method of obtaining a gel structure including a protein extract from a tissue, the method comprising:
- selecting a tissue for extraction of basement-membrane proteins;
- removing cells of the selected tissue to achieve a decellularized basement membrane free of cellular contaminants;
- extracting basement membrane components from the decellularized basement membrane;
- and
- gelling the extracted basement membrane.
A method of obtaining a gel structure including a protein extract from a tissue, the method comprising:
- selecting a tissue for extraction of its basement-membrane proteins;
- homogenizing the basement membrane in a salt solution containing a protease inhibitor;
- extracting basement membrane components of the homogenized basement membrane in a high molar urea solution;
- gelling the extracted basement membrane components in the urea to attain a gel structure by lowering the pH of the solution or adding cells to the solution.
Dependencies are inferred from the claim text.