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AssigneeTriPath Imaging, Inc.
InventorsDouglas P. Malinowski, Timothy J. Fischer, Adriann J. Taylor
Estimating the term…
Compositions and methods for diagnosing high-grade cervical disease in a patient sample are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to MCM6 or MCM7. Monoclonal antibodies having the binding characteristics of an MCM6 or MCM7 antibody of the invention are further provided. Hybridoma cell lines that produce an MCM6 or MCM7 monoclonal antibody of the invention are also disclosed herein. The compositions find use in practicing methods for diagnosing high-grade cervical disease comprising detecting overexpression of MCM6, MCM7, or both MCM6 and MCM7 in a cervical sample from a patient. Kits for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for an MCM6 or an MCM7 epitope and methods of using these polypeptides in the production of antibodies are also encompassed by the present invention.
A monoclonal antibody that is capable of specifically binding to MCM7, or a variant or fragment thereof, wherein the antibody is selected from the group consisting of:
The hybridoma cell line 2E6.2, deposited with the ATCC as Patent Deposit No. PTA-6669.
A monoclonal antibody that is capable of specifically binding to MCM6, or a variant or fragment thereof, wherein the antibody is selected from the group consisting of:
The hybridoma cell line 9D4.3, deposited with the ATCC as Patent Deposit No. PTA-6911.
A kit comprising at least at least two monoclonal antibodies, wherein a first antibody is the MCM7 monoclonal antibody produced by the hybridoma cell line 2E6.2, deposited with the ATCC as Patent Deposit No. PTA-6669, and a second antibody is the MCM6 monoclonal antibody produced by the hybridoma cell line 9D4.3, deposited with the ATCC as Patent Deposit No. PTA-6911.
An isolated polypeptide comprising an epitope for binding an MCM7 monoclonal antibody, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of:
An isolated polypeptide comprising an epitope for binding an MCM6 monoclonal antibody, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of:
Dependencies are inferred from the claim text.