Methods for modulating mannose content of recombinant proteins

A method of producing a composition of recombinant antibody, or an antigen-binding fragment thereof, having low-mannose content comprising culturing a host-cell which expresses the recombinant antibody or antigen-binding fragment thereof in a culture medium having an osmolality of about 600 mOsm/Kg or less.

A method of producing a composition of recombinant human monoclonal antibody, or antigen-binding fragment thereof, that binds IL-15, comprising culturing a host-cell which expresses the antibody or antigen-binding fragment thereof in a culture medium having an osmolality of about 600 mOsm/Kg or less, wherein the antibody or antigen-binding fragment thereof comprises a light chain variable region comprising the amino acid sequence set forth in SEQ ID NO:4, or conservative amino acid substitutions thereof, and a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:2, or conservative amino acid substitutions thereof.

A composition comprising an isolated antibody or an antigen-binding fragment thereof having low-mannose content.

A composition comprising an isolated human monoclonal antibody that binds IL-15, or an antigen-binding fragment thereof, having low-mannose content.

A composition of a human monoclonal antibody having low-mannose content, said antibody comprising a light chain variable region comprising the amino acid sequence set forth in SEQ ID NO:4, or an amino acid sequence of at least 90% or at least 95% identity thereto, or conservative amino acid substitutions thereof, and a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:2, or an amino acid sequence of at least 90% or at least 95% identity thereto, or conservative amino acid substitutions thereof.

A composition of a human monoclonal antibody having low-mannose content, said antibody comprising a light chain variable region comprising one or more CDRs comprising amino acid sequences set forth in SEQ ID NOs:8-10, or an amino acid sequence of at least 90% or at least 95% identity thereto, or conservative amino acid substitutions thereof, and a heavy chain variable region comprising one or more CDRs comprising amino acid sequences set forth in SEQ ID NOs:5-7, or an amino acid sequence of at least 90% or at least 95% identity thereto, or conservative amino acid substitutions thereof.

A method of detecting and/or quantitating the high-mannose content of a glycoprotein in a sample, said method comprising subjecting said sample to an endoglycosidase digestion followed by separating the digested glycoproteins by electrophoresis.