Novel cell lines, and methods of preparation and use thereof
OwnerProbal Banerjeeinventor
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Abstract
The present invention provides, in some embodiments, hippocampal neuron-derived cell lines that display voltage-gated calcium channels of various types, including the N-type channels. Such mixed calcium channel activity is similar to that observed in the hippocampal neurons from which they were originally derived. The cells also can be differentiated into neurons and contain many of the important hippocampal neuron proteins. The invention further provides methods for using the cells.
Claims51
A method for inducing voltage gated calcium current activity in a mammalian hippocampal neuron-derived cell comprising the steps of:
- providing a mammalian hippocampal neuron-derived cell;
- and
- transfecting said cell with a recombinant construct comprising a nucleotide sequence encoding a P-type ATPase.
A method for identifying a compound that inhibits calcium channels in hippocampal neurons comprising the steps of:
- contacting one or more candidate compounds with an ATPase-transformed mammalian hippocampal neuron-derived cell;
- and
- measuring the ability of the compound or compounds to inhibit activity of said channels.
A method for identifying a compound that stimulates a receptor that inhibits calcium channels in hippocampal neurons comprising the steps of:
- contacting one or more candidate compounds with an ATPase transformed mammalian hippocampal neuron-derived cell, wherein said cell expresses at least one receptor that modulates said calcium channels;
- and
- measuring the ability of the compound or compounds to inhibit activity of said channels.
An ATPase transformed mammalian hippocampal neuron-derived cell having voltage gated calcium current activity.
An ATPase II-transformed mammalian hippocampal neuron-derived cell having voltage gated calcium current activity.
An ATPase transformed mammalian hippocampal neuron-derived cell, wherein said cell comprises N-type calcium channels.
An ATPase II transformed mammalian hippocampal neuron-derived cell, wherein said cell comprises N-type calcium channels.
An ATPase II transformed mammalian hippocampal neuron-derived cell having voltage gated calcium current activity produced by a method comprising transfecting a HN2 cell with a recombinant construct comprising an expression vector;
- said expression vector comprising a nucleotide sequence encoding a murine ATPase II;
- wherein said nucleotide sequence has the sequence of GeneBank accession number U75321.
The cell line HN2A12.
The cell line HN2A22.
Dependencies are inferred from the claim text.