Closed cell culture system

A combination of a perfusion introduction tube, a perfusion discharge tube, and a disc used for a closed cell culture system characterized in that a defection of a tissue on the body surface or inside the body is tightly sealed from the outside to form a closed environment free from the invasion of bacteria, and a solution appropriate for cell culture is circulated or perfused in the tissue defection thus sealed to thereby regenerate the defective tissue, wherein said perfusion introduction tube introduces said cell culture solution in said defection of a tissue on the body surface, said perfusion discharge tube discharges the introduced fluid, and said disc comprises disc 1 and disc 2, said perfusion introduction tube being provided in the disc 2, perfusion from said perfusion introduction tube being introduced into said defection of a tissue via said disc 2, and said perfusion discharge tube being provided in said disc 1, whereby perfusion filling said defection of a tissue passes through an airspace between said disc 1 and disc 2 and is discharged from said perfusion discharge tube, thereby completing perfusion of cell culture solution into said defection of a tissue.