Listeria-induced immunorecruitment and activation, and methods of use thereof
AssigneesCerus Corporation, The Johns Hopkins University
InventorsDrew Pardoll, Richard Schulick, Keith Bahjat, Dirk Brockstedt, Thomas Dubensky, Martin Giedlin, Kiyoshi Yoshimura, Ajay Jain
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Abstract
Provided are reagents and methods for administering an attenuated bacterium for use in treating a cancerous or infectious condition. Reagents and methods for administering an attenuated bacterium for use in inducing an immune response against a tumor, cancer cell, or infective agent are further provided. Also provided are methods of diagnosis and kits.
Claims55
A method for treating a mammal having a cancerous or non-listerial infectious condition, wherein the cancerous or infection condition is in the liver of the mammal, comprising administering to the mammal an effective amount of a metabolically active, attenuated Listeria, wherein the Listeria does not comprise a nucleic acid encoding a non-listerial antigen capable of stimulating a specific immune response against the condition, and wherein the attenuated Listeria is administered to the mammal in multiple doses.
A method for inducing an immune response against a cancer cell, tumor, or non-listerial infective agent in a mammal, wherein the mammal comprises the cancer cell, tumor, or non-listerial infective agent in its liver, comprising administering to the mammal an effective amount of a metabolically active, attenuated Listeria, wherein the Listeria does not comprise a nucleic acid encoding a non-listerial antigen capable of stimulating a specific immune response against the condition, wherein the attenuated Listeria is administered to the mammal in multiple doses, and wherein the attenuated Listeria is one or both of:
- a. not administered orally to the mammal, or
- b. administered as a composition that is at least 99% free of other types of bacteria.
A method for inducing an immune response against a cancer cell, tumor, or non-listerial infective agent in a mammal, wherein the mammal comprises the cancer cell, tumor, or non-listerial infective agent in its liver, comprising administering to the mammal an effective amount of a metabolically active, attenuated Listeria, wherein the Listeria does not comprise a nucleic acid encoding a non-listerial antigen capable of stimulating a specific immune response against the condition, wherein the attenuated Listeria is administered to the mammal in multiple doses, and wherein the attenuated Listeria is one or both of:
- a. administered in a pharmaceutical composition;
- or
- b. a non-naturally occurring strain.
A method for treating a mammal having a cancerous or non-listerial infectious condition, wherein the cancerous or infectious condition is in the liver of the mammal, comprising administering to the mammal an effective amount of a metabolically active, attenuated Listeria, wherein the Listeria does not comprise a nucleic acid encoding a non-listerial antigen capable of stimulating a specific immune response against the condition, and wherein the Listeria is administered to the mammal in the absence of a separately generated, vaccine-induced immune response to the cancerous or infectious condition in the mammal.
Dependencies are inferred from the claim text.