Collagen producing plants and methods of generating and using same
AssigneeCollPlant Ltd.
InventorsOded Shoseyov, Hanan Stein
Life of this patent
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Abstract
A method of producing collagen in a plant and plants producing collagen are provided. The method is effected by expressing in the plant at least one type of a collagen alpha chain in a manner enabling accumulation of the collagen alpha chain in a subcellular compartment devoid of endogenous P4H activity, thereby producing the collagen in the plant.
Claims37
A method of producing collagen in a plant or an isolated plant cell comprising targeting to a vacuole of the plant or the isolated plant cell at least one type of a collagen chain and an exogenous P4H so as to allow hydroxylation of said at least one type of said collagen chain by said exogenous P4H and not by an endogenous P4H of the plant or isolated, plant cell, thereby producing the collagen in the plant.
A genetically modified plant or isolated plant cell comprising in a vacuole thereof:
- (i) at least one type of a collagen chain;
- and
- (ii) an exogenous P4H.
A plant system comprising:
- a first genetically modified plant comprising in a vacuole thereof:
- (i) a collagen alpha 1 chain;
- and
- (ii) an exogenous P4H;
- and
- a second genetically modified plant comprising in a vacuole thereof:
- (i) a collagen alpha 2 chain;
- and
- (ii) an exogenous P4H.
A plant system comprising:
- a first genetically modified plant comprising in a vacuole thereof:
- (i) a collagen alpha 1 chain;
- and
- (ii) a collagen alpha 2 chain;
- and
- a second genetically modified plant comprising in a vacuole thereof an exogenous P4H.
A nucleic acid construct comprising a polynucleotide encoding a human P4H attached to a vacuole targeting signal, said polynucleotide being positioned under the transcriptional control of a promoter functional in plant cells.
A genetically modified plant or isolated plant cell being capable of accumulating collagen having a temperature stability at 42° C.
Dependencies are inferred from the claim text.