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US 2007/0186309 A1Published application
Plant viral movement protein genes
AssigneeE. I. Du Pont de Nemours and Company
InventorsEnno Krebbers, Zude Weng, Rebecca Cahoon
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a viral movement protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the viral movement protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the viral movement protein in a transformed host cell.
Claims15
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) a first nucleotide sequence encoding a polypeptide of at least 129 amino acids that has at least 95% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 8,10,12, 14, 16, 18, 20, 22, 24, 26, 28, 30 and 32, or
- (b) a second nucleotide sequence comprising a complement of the first nucleotide sequence.
2-23. (canceled)
An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a viral movement polypeptide, wherein the polypeptide has an amino acid sequence of at least 85% sequence identity, based on the Clustal method of alignment with multiple alignment default parameters of GAP PENALTY=10 and GAP LENGTH PENALTY=10, and pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, when compared to SEQ ID NO:4, or
- (b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
Dependencies are inferred from the claim text.