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US 2007/0186296 A1Published application
Cloning, characterization, and application of tnfrsf19 in neurological disorders
AssigneeWyeth
InventorsYing Gao, Huai-Ping Ling, Andrew Wood, Kodangattil Sreekumar
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Abstract
TNFRSF19 nucleic acids and proteins, TNFRSF19 binding partners and modulators, and methods of using the same.
Claims20
An isolated TNFRSF19 nucleic acid comprising:
- (a) an open reading frame encoding a TNFRSF19 protein comprising an amino acid sequence at least 91% identical to SEQ ID NO:2 or 4;
- (b) an open reading frame encoding a TNFRSF19 protein comprising an amino acid sequence of SEQ ID NO:2 or 4;
- (c) a nucleotide sequence at least 93% identical to SEQ ID NO:
- 1 or 3;
- (d) a nucleotide sequence of SEQ ID NO:1 or 3;
- (e) a nucleic acid that specifically hybridizes to the complement of SEQ ID NO:1 or 3 under stringent hybridization conditions;
- or
- (f) a nucleotide sequence that is the complement of any one of (a)-(e).
5 more elements
An isolated TNFRSF19 protein comprising:
- (a) an amino acid sequence at least 91% identical to SEQ ID NO:2 or 4;
- or
- (b) an amino acid sequence of SEQ ID NO:2 or 4.
A method for identifying a binding partner of a TNFRSF19 protein, the method comprising the steps of:
- (a) providing a TNFRSF19 protein comprising (i) an amino acid sequence at least 91% identical to SEQ ID NO:2 or 4;
- or (ii) an amino acid sequence of SEQ ID NO:2 or 4;
- (b) providing a one or more test agents to the TNFRSF19 protein under conditions sufficient for binding;
- (c) assaying binding of a test agent to the isolated TNFRSF19 protein;
- and
- (d) selecting a test agent that demonstrates specific binding to the TNFRSF19 protein.
3 more elements
A method for identifying agents for disinhibition of neurite outgrowth comprising the steps of:
- (a) providing a TNFRSF19 protein under conditions for formation of a receptor complex comprising NgR, wherein the TNFRSF19 protein comprises (i) an amino acid sequence at least 91% identical to SEQ ID NO:2 or 4;
- or (ii) an amino acid sequence of SEQ ID NO:2 or 4;
- (b) providing one or more test agents or a control agent to the TNFRSF19 protein and NgR of (a);
- (c) allowing sufficient time for formation of a receptor complex;
- (d) assaying (i) formation of a receptor complex, (ii) capacity of the receptor complex of (c) to activate RhoA;
- or (iii) capacity of the receptor complex of (c) to inhibit neurite outgrowth;
- and
- (e) selecting a test agent that shows (i) altered formation of a receptor complex in the presence of the agent as compared to a control agent, (ii) altered capacity of the receptor complex of (c) to activate RhoA when in the presence of the test agent as compared to a control agent;
- or (iii) altered capacity of the receptor complex of (c) to inhibit neurite outgrowth when in the presence of the test agent as compared to a control agent.
6 more elements
Dependencies are inferred from the claim text.