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US 2007/0186291 A1Published application
Method for generating overexpression of alleles in genes of unknown function
AssigneeThe Regents of the University of California
InventorsEthan Bier, Annabel Guichard, Shaila Srinivasan
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Abstract
Methods for generating and using novel overexpression activity alleles of a gene in any organism, especially Drosophilia, are provided. Such alleles may be utilized in screening assays, and used to generate dominant-negative forms of bacterial toxins.
Claims9
A method for generating in an organism a novel overexpression activity (NOVA) allele of a gene of unknown function, comprising:
- misexpressing a wild-type copy of a gene by known methods;
- determining the phenotypic consequence of the misexpressing, namely transgene-x;
- mutagenizing male organisms carrying the transgene-x;
- activating global or restricted expression by mating en masse to relevant female strains;
- screening progeny for new NOVA dominant phenotypes resulting from the expression;
- and
- crossing NOVA mutants to appropriate balancer females to establish stable stocks.
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