Diabetogenic epitopes
OwnerFraser Scott, Amanda MacFarlane +2inventors
InventorsFraser Scott, Amanda MacFarlane, Karolina Burghardt, Majid Mojibian
Life of this patent
Estimating the term…
Abstract
The present invention provides nucleotide and amino acid sequences of diabetogenic epitopes, and proteins comprising diabetogenic epitopes. Also provided are kits comprising diabetogenic epitopes, methods of identifying subjects comprising antibodies to diabetogenic epitopes and foodstuffs modified to remove or reduce diabetogenic epitopes or proteins comprising diabetogenic epitopes. Diabetogenic epitopes and proteins comprising diabetogenic epitopes from isoforms of gliadin proteins.
Claims35
An amino acid sequence comprising a diabetogenic epitope selected from the group consisting of isoforms of gliadin proteins or Glb1.
A nucleotide sequence encoding a diabetogenic epitope from isoforms of gliadin proteins or Glb1.
A nucleotide sequence complementary to a sequence encoding a diabetogenic epitope or a portion thereof.
An isolated antibody capable of binding to Glb1 or one or more isoforms of gliadin proteins.
An isolated antibody capable of binding to a diabetogenic epitope of Glb1, or one or more isoforms of gliadin proteins.
A kit comprising one or more of 1) a diabetogenic epitope, 2) a protein or peptide comprising a diabetogenic epitope, 3) a non-protein carrier or macromolecule comprising the diabetogenic epitope, 4) a support comprising the diabetogenic epitope, 5) a diabetogenic epitope attached to a non-covalent association agent, 6) a nucleotide sequence encoding a diabetogenic epitope or peptide or protein comprising the diabetogenic epitope, 7) a nucleotide sequence complementary to a nucleotide sequence encoding a diabetogenic epitope, 8) a nucleotide sequence complementary to a portion of a nucleotide sequence encoding a diabetogenic protein, or a combination thereof.
A method of screening foodstuffs to identify proteins in the foodstuff which are antigenic/immunogenic in a subject, or group of subjects comprising a pathological condition, the method comprising the steps of:
- a) processing the foodstuff to produce separated proteins, and;
- b) screening the separated proteins from step a) with an antibody containing mixture derived from one or more subjects having the pathological condition to identify proteins that are antigenic/immunogenic in the subject and that are present in the foodstuff.
A method of screening foodstuffs to identify antigenic/immunogenic proteins common in at least two subjects, or groups of subjects wherein each subject or group of subjects comprise different pathological conditions, the method comprising the steps of
- a) processing the foodstuff to produce separated proteins;
- b) screening the separated proteins from step a) with a first antibody containing mixture derived from one or more subjects having a first pathological condition;
- c) screening the separated proteins from step a) with a second antibody containing mixture derived from one or more subjects having a second pathological condition;
- d) comparing proteins binding to the first antibody containing mixture with proteins binding to the second antibody mixture to identify proteins common in at least two subjects, or groups of subjects with different pathological conditions, the proteins also present in the foodstuff.
A foodstuff modified to reduce or eliminate one or more diabetogenic epitopes or proteins comprising diabetogenic epitopes.
A method of screening a subject for reactivity toward one or more food proteins comprising the steps of a) isolating blood from said subject;
- b) optionally purifying one or more components from said blood;
- c) contacting said blood with one or more food proteins or fragments thereof and d) measuring a biological response thereto.
Dependencies are inferred from the claim text.