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AssigneePaul Scherrer Institut
InventorsRoger Schibli, Albert Stichelberger, Robert Waibel, August Schubiger
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The present invention relates to a method for radioactive labeling of a protein or peptide, by providing a protein or peptide having at least one glutamine or lysine residue; adding a metal chelating agent having at least one lysine or glutamine residue, respectively, which metal chelating agent is optionally complexed with a radioactive or paramagnetic metal; reacting the protein or peptide and metal chelating agent in the presence of a transglutaminase to obtain a protein or peptide with a metal chelating group covalently bound thereto, and optionally complexing the metal chelating group with a radioactive or paramagnetic metal. The invention also relates to proteins and peptides thus labeled and to proteins and peptides that have been coupled to a metal chelating agent but not yet labeled.
A method for radioactive labeling a protein or peptide, the method comprising the steps of:
A method for radioactive labeling of a protein or peptide, the method comprising the steps of:
(canceled)
(canceled)
A bifunctional transition metal chelating agent comprising a metal chelating moiety and a lysine or glutamine side chain.
A radioactivey labeled bifunctional metal chelating agent comprising the general formula:
The radioactively labeled bifunctional metal chelating agent according to claim 24, wherein M(CO)3 in the formula is replaced by another radioactive or paramagnetic transition metal.
Dependencies are inferred from the claim text.