Microbial production of l-ascorbic acid
AssigneeDsm IP Assets B.V.
InventorsAlan Berry, Connie Lee, Anne Mayer, Masako Shinjoh
Life of this patent
Estimating the term…
Abstract
The present invention discloses an isolated polynucleotide molecule derived from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1. The present invention further relates to a process for the production of L-ascorbic acid in high yield, in particular a process using resting cells of a microorganism able to convert given carbon sources into vitamin C. The thus obtained vitamin C may be further processed by purification and/or separation steps.
Claims42
An isolated polynucleotide molecule derivable from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1.
A process for the production of vitamin C comprising converting a substrate into vitamin C in a medium comprising resting cells of a microorganism.
Dependencies are inferred from the claim text.