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US 2007/0184532 A1Published application
Method for Producing L-Histidine Using Bacteria of Enterobacteriaceae Family
AssigneeAjinomoto Co., Inc.
InventorsElena Klyachko, Rustam Shakulov, Yuri Kozlov
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Abstract
A method is provided for producing L-histidine using bacterium of the Enterobacteriaceae family, wherein the L-amino acid productivity of said bacterium is enhanced by enhancing an activity of the transaldolase encoded by the talB gene.
Claims16
A method for producing L-histidine comprising
- A) cultivating a bacterium in a culture medium, and
- B) collecting L-histidine from the culture medium,
- wherein the bacterium is of the Enterobacteriaceae family and has been modified to enhance transaldolase activity by increasing the copy number of the transaldolase gene or by replacing a promoter sequence of the transaldolase gene with a stronger promoter sequence, wherein said transaldoase gene comprises a DNA which is 90% or more homologous to nucleotides 1-954 of SEQ ID No. 1 and encodes a protein which has transaldolase activity.
A method for producing L-histidine comprising
- A) cultivating a bacterium in a culture medium, and
- B) collecting L-histidine from the culture medium,
- wherein the bacterium is of the Enterobacteriaceae family and has been modified to enhance transaldolase activity, wherein the transaldolase is selected from the group consisting of
- a) a protein comprising the amino acid sequence of SEQ ID NO. 2, and
- b) a protein which has 90% or more homology to the amino acid sequence of SEQ ID NO. 2 and has the activity of transaldolase.
2 more elements
Dependencies are inferred from the claim text.