Gene encoding cyclododecanone monooxygenase
AssigneeNational Research Council of Canada
InventorsHiroaki Iwaki, Yoshie Hasegawa, Peter Lau
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Abstract
The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.
Claims19
An isolated DNA encoding a cyclododecanone monooxygenase (CDMO) having an amino acid sequence as set forth in SEQ ID NO:8, or an enzymatically active portion thereof.
An isolated DNA encoding a cyclododecanone monooxygenase (CDMO) or an enzymatically active portion thereof, the isolated DNA being characterized by the ability to hybridize specifically with the complement of the DNA represented in SEQ ID NO:7 under stringent hybridization conditions.
An isolated DNA coding for a cyclododecanone monooxygenase (CDMO), and containing:
- (a) a nucleic acid sequence as set forth in SEQ ID NO:7;
- (b) a sequence corresponding within the degeneracy of the genetic code to said nucleic acid sequence and coding for a cyclododecanone monooxygenase;
- or
- (c) a sequence hybridizing under stringent conditions with the complement of the sequence from (a) or (b), and still coding for cyclododecanone monooxygenase (CDMO).
An isolated DNA encoding a cyclododecanone monooxygenase (CDMO), or an enzymatically active portion thereof, said isolated DNA having SEQ ID NO:7.
A cyclododecanone monooxygenase (CDMO) having an amino acid sequence as set forth in SEQ ID NO:8.
A Pseudomonas putida strain HI-70 having accession number IDAC-180902.
Dependencies are inferred from the claim text.