Novel phytase and gene
OwnerAlissa Jourdan, Jennifer Radosevichinventors
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Abstract
The invention relates to a novel phytase excreted by an isolated fungal strain, nucleic acids encoding the phytase, prokaryotic and eukaryotic host cells transformed by the nucleic acids, and methods for producing the phytase.
Claims23
A phytase excreted by an isolated fungal strain ATCC accession no. SD5361.
An isolated nucleic acid which encodes a phytase having an activity profile wherein the activity of the phytase retains at least 30% of its maximum activity over a temperature range from about 80° C. to about 100° C. and which is at least 74.9% identical to SEQ ID No. 1 wherein the % identity is determined using a sequence comparison algorithm or by visual inspection.
A plasmid comprising an isolated DNA molecule which encodes a phytase which retains at least 30% of its maximum activity over a temperature range from about 80° C. to about 100° C. and which is at least 70% identical to SEQ ID No. 1 wherein the % identity is determined using a sequence comparison algorithm or by visual inspection.
An isolated nucleic acid which encodes a phytase having an activity profile wherein the activity of the phytase retains at least 30% of its maximum activity over a temperature range from about 80° C. to about 100° C. and which hybridizes to SEQ ID No. 1 under conditions of low stringency.
An isolated phytase protein which retains at least 30% of its maximum activity over a temperature range from about 80° C. to about 100° C. and comprising an amino acid sequence that is at least 70% identical to SEQ ID NO. 2 as determined by analysis with a sequence comparison algorithm or by visual inspection.
Dependencies are inferred from the claim text.