Method and apparatus for monitoring enzyme mixtures
OwnerFrederick Rowell, Latha Sundarinventors
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Abstract
The present invention provides a method for simultaneously detecting the presence of at least two enzymes in a sample. The method includes the steps of: i) providing a first substrate for a first enzyme, the first substrate being labeled with a first fluorophore; ii) providing a second substrate for a second enzyme, the second substrate being labeled with a second fluorophore; iii) exposing the labeled substrates to the sample to allow the first and second enzymes present in the sample to interact with respective first and second fluorophore-labeled substrates to form respective first and second fluorophore-labeled substrate fragments; and iv) detecting the presence of the fluorophore-labeled substrate fragments.
Claims22
A method for simultaneously detecting a presence of at least two enzymes in a sample, comprising the steps of:
- a.) providing a first substrate for a first enzyme, the first substrate being labeled with a first fluorophore;
- b.) providing a second substrate for a second enzyme, the second substrate being labeled with a second fluorophore;
- c.) exposing the labeled substrates to the sample to allow the first and second enzymes present in the sample to interact with respective first and second fluorophore-labeled substrates to form respective first and second fluorophore-labeled substrate fragments;
- and
- d.) detecting the presence of the fluorophore-labeled substrate fragments.
A vessel for use in simultaneous detection of a presence of at least two enzymes in a sample, comprising:
- a first substrate for a first enzyme, the first substrate being labeled with a first fluorophore;
- and
- a second substrate for a second enzyme, the second substrate being labeled with a second fluorophore.
An apparatus for use in simultaneous detection of at least two enzymes in a sample, comprising:
- a vessel,
- wherein the vessel comprises:
- a first substrate for a first enzyme, the substrate being labeled with a first fluorophore;
- and
- a second substrate for a second enzyme, the second substrate being labeled with a second fluorophore;
- and
- detecting means for detecting fluorescent substrate fragments.
An apparatus for use in simultaneous detection of at least two enzymes in a sample, comprising:
- a first vessel,
- wherein the first vessel comprises:
- a first substrate for a first enzyme, the substrate being labeled with a first fluorophore,
- wherein the first vessel is connectable to a second vessel, and
- wherein the second vessel comprises:
- a second substrate for a second enzyme, the substrate being labeled with a second fluorophore;
- and
- detecting means for detecting fluorescent substrate fragments.
Dependencies are inferred from the claim text.