Assay method and apparatus
AssigneeNalia Systems Ltd.
InventorsJeffrey McBride, Francis Gabriel, Peter Delves, John Fordham, Ian Cree, Keith Rawson
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Abstract
The present invention relates to an apparatus for use in an assay comprising: (a) at least one first receptacle comprising a fluid inlet and a fluid outlet, (b) a porous membrane, and (c) at least one analyte-specific binding agent, characterised in that said at least one analyte-specific binding agent is immobilised on the underside of said porous membrane relative to the fluid inlet; and to methods of performing said assay.
Claims36
An apparatus for use in an assay comprising:
- (a) at least one first receptacle comprising a fluid inlet and a fluid outlet;
- (b) a porous membrane;
- and
- (c) at least one analyte-specific binding agent,
- characterised in that said at least one analyte-specific binding agent is immobilised on the underside of said porous membrane relative to the fluid inlet.
(canceled)
(canceled)
22-43. (canceled)
45-53. (canceled)
A method of detecting an analyte comprising:
- (a) providing an apparatus comprising at least one first receptacle having a fluid inlet and a fluid outlet and having a porous membrane;
- (b) immobilising at least one analyte-specific binding agent in a spot on the underside of said porous membrane;
- (c) loading a sample to be tested into the at least one first receptacle;
- and
- (d) detecting binding of the analyte to the analyte-specific binding agent(s).
55-95. (canceled)
99-102. (canceled)
Dependencies are inferred from the claim text.