Dendritic chemiluminescent substrates
AssigneeApplied Biosystems, LLC
InventorAlison Sparks
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Abstract
Chemiluminescent substrate delivery systems comprising a conjugate a dendrimer and at least one chemiluminescent substrate are provided. The substrate delivery systems can also include a chemiluminescence enhancer. The dendrimer/chemiluminescent substrate conjugates can be used in kits including an enzyme capable of activating the chemiluminescent substrate to produce a peroxygenated intermediate that decomposes to produce light. The dendrimer/chemiluminescent substrate conjugates can be used in assays to detect the presence of an analyte (e.g., an enzyme, an antibody, an antigen or a nucleic acid) in a sample.
Claims45
A chemiluminescent substrate delivery system comprising:
- a dendrimer;
- and at least one enzymatically activated chemiluminescent substrate conjugated to the dendrimer.
8-15. (canceled)
A kit for conducting an assay to determine the presence or concentration of an analyte in a sample, wherein the kit comprises:
- a dendrimer comprising at least one enzymatically active chemiluminescent substrate moiety;
- and
- an enzyme capable of activating the chemiluminescent substrate to produce a peroxygenated intermediate that decomposes to produce light.
A method for detecting the presence of an analyte in a sample comprising:
- forming an enzyme complex between an enzyme and a substance capable of binding to the analyte;
- adding the enzyme complex to the sample;
- allowing the enzyme complex to bind with analyte present in the sample;
- adding a chemiluminescent delivery system to the sample;
- and
- measuring chemiluminescent emissions from the sample;
- wherein the chemiluminescent delivery system comprises a dendrimer and at least one enzymatically active chemiluminescent substrate conjugated to the dendrimer and wherein the amount of chemiluminescent emissions measured indicates the presence and/or the concentration of the analyte in the sample.
Dependencies are inferred from the claim text.