Card domain containing polypeptides, encoding nucleic acids, and methods of use
AssigneeThe Burnham Institute
InventorsJohn Reed, Frederick Pio, Adam Godzik, Christian Stehlik, Jason Damiano, Sug Lee, Vasco Oliveira, Hideki Hayashi, Kryzysztof Pawlowski
Life of this patent
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Abstract
The invention provides caspase recruitment domain (CARD)-containing polypeptides, CARD, NB-ARC, ANGIO-R, LRR and SAM domains therefrom, as well as encoding nucleic acid molecules and specific antibodies. The invention also provides related screening, diagnostic and therapeutic methods.
Claims23
1-8. (canceled)
An isolated CARD-containing polypeptide, or an NB-ARC domain therefrom, comprising an amino acid sequence at least 70% identical to the amino acid sequence set forth in SEQ ID NOS:
- 97 or 179.
A method of producing a CARD-containing polypeptide comprising expressing a cDNA encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:97 in vitro or in a cell under conditions suitable for expression of said polypeptide, wherein said cells are selected from the group consisting of bacteria cells, yeast cells, plant cells, animal cells, mammalian cells and insect cells.
A method for identifying a nucleic acid molecule encoding a CARD-containing polypeptide, said method comprising:
- contacting a sample containing nucleic acids with an oligonucleotide comprising at least 15 contiguous nucleotides of a nucleic acid encoding SEQ ID NO:97, wherein said contacting is effected under high stringency hybridization conditions, and identifying a nucleic acid molecule which hybridizes thereto.
A method of altering the level of a biochemical process modulated by a CARD-containing polypeptide, comprising the steps of:
- (a) introducing a nucleic acid molecule encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:97 into a cell;
- and
- (b) expressing said nucleic acid molecule in said cell, whereby the expression of said nucleic acid alters the level of a biochemical process modulated by a CARD-containing polypeptide.
A composition comprising a compound selected from the group consisting of a CARD-containing polypeptide encoded by the amino acid sequence of SEQ ID NO:97, a functional fragment therefrom, and an anti-CARD antibody;
- and a pharmaceutically acceptable carrier.
A chimeric polypeptide comprising a domain encoded by SEQ ID NO:
- 180.
A method of identifying an effective agent that modulates an activity of a NB-ARC domain of a CARD-containing polypeptide, comprising the steps of:
- (a) contacting a polypeptide comprising an NB-ARC domain encoded by SEQ ID NO:
- 180 with an agent known or suspected of modulating an activity of an NB-ARC domain;
- and
- (b) measuring the activity of the NB-ARC domain, whereby an increase or decrease of said activity identifies said agent as an agent that modulates the activity of the NB-ARC domain of said CARD-containing polypeptide;
- wherein the activity of the NB-ARC domain of said CARD-containing polypeptide is selected from homo-oligomerization, hetero-oligomerization, nucleotide hydrolysis, and nucleotide binding.
Dependencies are inferred from the claim text.