Method for hybridizing nucleic acids and hybridization apparatus
AssigneeCanon Kabushiki Kaisha
InventorKoji Nozato
Life of this patent
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Abstract
A method for hybridizing a target nucleic acid and a probe nucleic acid that can specifically bind to the target nucleic acid and that is immobilized on a substrate includes a first reaction step of allowing the target nucleic acid contained in a sample solution to react with the probe nucleic acid, a recovery step of recovering the sample solution after the first reaction step, a heating step of heating the recovered sample solution to the denaturation temperature of the target nucleic acid or a higher temperature, and a second reaction step of allowing the target nucleic acid contained in the sample solution after the heating step to react with the probe nucleic acid.
Claims20
A method for hybridizing a target nucleic acid and a probe nucleic acid that can specifically bind to the target nucleic acid and that is immobilized on a substrate, comprising:
- a first reaction step of allowing the target nucleic acid contained in a sample solution to react with the probe nucleic acid;
- a recovery step of recovering the sample solution after the first reaction step;
- a heating step of heating the recovered sample solution to the denaturation temperature of the target nucleic acid or a higher temperature;
- and
- a second reaction step of allowing the target nucleic acid contained in the sample solution after the heating step to react with the probe nucleic acid.
A hybridization apparatus for hybridizing a target nucleic acid and a probe nucleic acid that can specifically bind to the target nucleic acid and that is immobilized on a substrate, comprising:
- a setting section for setting the substrate;
- a supply path for supplying the substrate with a sample solution containing the target nucleic acid;
- a recovery mechanism for recovering the supplied sample solution;
- and
- a heater for heating the sample solution recovered by the recovery mechanism to the denaturation temperature or higher,
- wherein the recovery mechanism is configured so as to again supply the heated sample solution to the substrate.
A cartridge in which a probe nucleic acid that can specifically bind to a target nucleic acid is immobilized, comprising:
- a reaction chamber for accommodating the probe nucleic acid;
- a supply port for supplying the reaction chamber with a sample solution containing the target nucleic acid;
- and
- a recovery section for recovering the sample solution supplied to the reaction chamber.
An analysis apparatus for hybridizing a labeled target nucleic acid and a probe nucleic acid that can specifically bind to the target nucleic acid and that is immobilized on a substrate and for detecting a label present on the substrate comprising:
- a setting section for setting the substrate;
- a supply path for supplying the substrate with a sample solution containing the target nucleic acid;
- a recovery mechanism for recovering the sample solution;
- a heater for heating the sample solution recovered by the recovery mechanism to the denaturation temperature or higher;
- and
- a detector for detecting the label,
- wherein the recovery mechanism is configured so as to again supply the heated sample solution to the substrate.
Dependencies are inferred from the claim text.