Back to results
US 2007/0184478 A1Published application
Process for detecting a plurality of target nucleic acids
OwnerWalter Gumbrecht, Jorn Mosner +2inventors
InventorsWalter Gumbrecht, Jorn Mosner, Manfred Stanzel, Christian Zilch
Life of this patent
Estimating the term…
Abstract
A process and a kit for detecting a plurality of target nucleic acids are disclosed. In at least one embodiment, the process and/or kit includes using primers coupled to a semi-solid phase support.
Claims20
A process for detecting a plurality of target nucleic acids, comprising:
- (i) providing a plurality of primer pairs including, in each case, a first and a second oligonucleotide primer suitable for amplifying said target nucleic acids, with a plurality of said first primers being coupled to a semi-solid phase support and with the second primer, in each case, being free;
- (ii) providing a solution including a target nucleic acid to be detected,
- (iii) contacting the primer pairs of (i) with the solution of (ii) and conducting an amplification reaction under conditions in which the target nucleic acid is amplified;
- (iv) denaturing the double-stranded amplification products of (iii) to give single strands;
- (v) removing the semi-solid phase support with at least one of single-stranded amplification products coupled thereto and non-extended primers coupled thereto;
- and
- (vi) detecting the target nucleic acids.
4 more elements
A kit for detecting a plurality of target nucleic acids, comprising:
- a plurality of primer pairs including, in each case, a first and a second oligonucleotide primer suitable for amplifying said target nucleic acid, with a plurality of said first primers being coupled to a semi-solid phase support and the second primer, in each case, being free.
Dependencies are inferred from the claim text.