Biomarkers for liver fibrotic injury
AssigneesIndustrial Technology Research Institute (ITRI), National Health Research Institutes (NHRI)
InventorsHui-Chu Hsieh, Tzu-Ling Tseng, Li-Jen Su, Chi-Ying Huang, Shih-Lan Hsu
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Abstract
The present invention provides a method for detecting liver fibrotic injury, including fibrosis and/or cirrhosis, by assaying biological samples for differential expression of at least one gene encoding a protein chosen from SEQ ID NO: 1-SEQ ID NO: 63 and human orthologs thereof, wherein differential expression of at least one gene suggests the presence of liver fibrosis or cirrhosis. The invention also provides a kit containing nucleic acid probes or antibodies for detecting liver fibrosis and/or cirrhosis by assaying the differential expression of proteins encoded by SEQ ID NO: 1-SEQ ID NO: 63 and human orthologs thereof.
Claims19
A method of detecting liver fibrosis and/or cirrhosis comprising:
- obtaining a biological sample from a patient;
- and
- assaying the sample for differential expression of at least one gene encoding a protein chosen from SEQ ID NO:
- 1-SEQ ID NO:
- 63 and human orthologs thereof;
- wherein the differential expression of at least one gene suggests the presence of liver fibrosis and/or cirrhosis.
A method of detecting liver fibrosis and/or cirrhosis comprising:
- obtaining a biological sample from a patient;
- and
- assaying the sample for differential expression of at least one gene encoding a protein from a sequence comprising SEQ ID NO:
- 1 to SEQ ID NO:
- 120;
- wherein the differential expression of at least one gene suggests the presence of liver fibrosis and/or cirrhosis.
A method of identifying a compound that decreases the differential expression of at least one gene encoding a protein chosen from SEQ ID NO:
- 7-SEQ ID NO:
- 23 and SEQ ID NO:
- 42-SEQ ID NO:
- 63 and human orthologs thereof comprising:
- providing a cell expressing at least one gene encoding a protein chosen from SEQ ID NO:
- 7-SEQ ID NO:
- 23 and SEQ ID NO:
- 42-SEQ ID NO:
- 63 and human orthologs thereof;
- contacting the cell with a test compound;
- and
- determining whether the differential expression of the at least one gene is decreased in the presence of the test compound, wherein the decreased differential expression is an indication of halting or reversing liver fibrosis and/or cirrhosis.
A method of identifying a compound that increases the differential expression of at least one gene encoding a protein chosen from SEQ ID NO:
- 1-SEQ ID NO:
- 6, and SEQ ID NO:
- 24-SEQ ID NO:
- 41 and human orthologs thereof comprising:
- providing a cell expressing at least one gene encoding a protein chosen from SEQ ID NO:
- 1-SEQ ID NO:
- 6 and SEQ ID NO:
- 24-SEQ ID NO:
- 41 and human orthologs thereof;
- contacting the cell with a test compound;
- and
- determining whether the differential expression of the at least one gene is increased in the presence of the test compound, wherein the increased differential expression is an indication of halting or reversing liver fibrosis and/or cirrhosis.
A method of diagnosing liver fibrosis and/or cirrhosis comprising:
- obtaining a first biological sample from a first subject without liver fibrosis and/or cirrhosis;
- obtaining a second biological sample from a second subject known or suspected of having liver fibrosis and/or cirrhosis;
- and
- determining an amount of at least one protein chosen from SEQ ID NO:
- 1 to SEQ ID NO:
- 120 in said first and second biological samples;
- wherein a difference between the amount of said protein present in the first biological sample from said first subject and the amount of said protein present in the second biological sample from said second subject indicates that the second subject has liver fibrosis and/or cirrhosis.
Dependencies are inferred from the claim text.