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US 2007/0184474 A1Published application
Process for amplifying DNA
AssigneeNichirei Corporation
InventorsTakeshi Koizumi, Yoko Hamano, Satoshi Yamamoto
Life of this patent
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Abstract
The present invention has an object of providing a process for amplifying DNA. The present invention provides a process for amplifying DNA, comprising providing a primer in which a compound such as LC-Red 705 is added to the 5′ terminus; and amplifying said target DNA fragment via PCR using said PCR primer, in which annealing is carried out at the temperature higher than normal PCR reaction, and/or with the annealing time shorter than normal PCR reaction
Claims5
A method for amplifying a target DNA fragment comprising:
- providing a PCR primer that comprises a compound at the 5′ terminus, said compound selected from a group consisting of LC-Red 705, an amino group, a phosphate group, DIG, DNP, TAMRA, Texas-Red, ROX, XRITC, rhodamine, LC-Red 640, a mercapto group, psoralen, cholesterol, FITC, 6-FAM, TET, cy3, cy5, BODIPY 564/570, BODIPY 500/510, BODIPY 530/550, BODIPY 581/591;
- and
- amplifying said target DNA fragment via PCR using said PCR primer, in which annealing of said primer to said DNA fragment is carried out at the temperature higher than normal PCR reaction, and/or with the annealing time shorter than normal PCR reaction.
Dependencies are inferred from the claim text.