Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna
AssigneeToagosei Co., Ltd.
InventorsMasaji Okamoto, Kenichi Tanaka
Life of this patent
Estimating the term…
Abstract
The method of purifying environmental DNA and the method of screening for a gene encoding a functional protein from environmental DNA are provided. DNA from a environmental sample is purified by recovering DNA from an environmental sample and allowing the thus recovered DNA to be subjected to gel filtration chromatography and ion exchange chromatography. In addition, a display library of DNA derived from an environmental sample is created so that screening is carried out in terms of the interaction of the above display library with ligands. Thus, screening of protein-encoding genes can be carried out.
Claims16
A method of screening for protein-encoding genes, comprising creating a display library of environmental DNA derived from an environmental sample and carrying out screening based on the interaction of the library with ligands.
A method of purifying environmental DNA derived from an environmental sample, comprising recovering DNA from an environmental sample and allowing the DNA to be subjected to gel filtration chromatography and/or ion exchange chromatography.
Dependencies are inferred from the claim text.