RNA probes
OwnerAzeddine Si-Ammour, Todd Blevins +1inventors
InventorsAzeddine Si-Ammour, Todd Blevins, Frederick Meins
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Abstract
The invention is based in part on the generation of a double stranded RNA molecule substantially covering the whole transcribed region of a gene, and cleaving this using an RNA endonuclease to generate small RNA molecules which are already or may be subsequently labelled. The invention provides small labelled ribonucleic acid (RNA) fragments for use as probes to detect potentially small interfering ribonucleic acid (siRNA) fragments produced in vivo. The invention also provides uses of said small labelled RNA fragments and kits suitable for preparing said small labelled RNA fragments.
Claims15
A method of generating small labelled ribonucleic acid (RNA) fragments, comprising the steps of:
- (a) providing a large unlabelled double stranded RNA fragment;
- (b) cleaving said large double stranded RNA fragment using an RNA endonuclease in order to generate small RNA fragments;
- and
- (c) labelling said small RNA fragments in order to generate said small labelled RNA fragments.
A method of generating small labelled RNA fragments, comprising the steps of:
- (a) providing a large labelled double stranded RNA fragment;
- and
- (b) cleaving said large double stranded RNA fragment using an RNA endonuclease in order to generate said small labelled RNA fragments.
A kit for use in generating small labelled ribonucleotide fragments, the kit comprising a cloning vector for use in generating a large double stranded RNA fragment:
- and an RNA endonuclease which is capable of cleaving said large double stranded RNA fragment in order to generate small labelled or unlabelled RNA fragments.
Dependencies are inferred from the claim text.