Apparatus and method for solid-phase kinetic analysis of templated elongation reactions
OwnerZachary Burton, Yuri Nedialkov +1inventors
InventorsZachary Burton, Yuri Nedialkov, Xueqian Gong
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Abstract
Disclosed is an elongation reaction system that includes a membrane compatible with binding an elongation complex and an elongation complex compatible with binding the membrane. The elongation complex includes the biological template (e.g., DNA or RNA), a polymerizing agent (e.g., RNA polymerase, DNA polymerase, or a ribosome), and a primer transcript or polypeptide. Further, disclosed is an apparatus and method for solid-phase kinetic analysis of templated elongation reactions.
Claims23
An immobilized elongation reaction system comprising:
- a membrane and an elongation complex;
- the membrane being compatible with binding the elongation complex;
- and
- the elongation complex being compatible with binding the membrane, the elongation complex including a template, a polymerizing agent, and a primer.
An apparatus for millisecond kinetic analysis of a templated elongation reaction comprising:
- a membrane compatible with anchoring an elongation complex;
- a reaction chamber adapted to receive the membrane;
- at least two reagents;
- and
- a feed-line interconnecting the reagents and the reaction chamber.
A method for millisecond kinetic analysis of a templated elongation reaction comprising:
- providing an apparatus for millisecond kinetic analysis including a membrane compatible with binding an elongation complex, a reaction chamber, at least two reagents, and a feed-line interconnecting the reagents and the reaction chamber;
- binding the elongation complex to the membrane, the elongation complex including a template, a polymerizing agent, and a primer;
- moving the membrane into the reaction chamber;
- and
- independently flowing at least two reagents over the elongation complex to permit elongation of the primer and quenching of the elongation complex.
Dependencies are inferred from the claim text.