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US 2007/0184456 A1Published application
Use of microfluidic systems in the detection of target analytes using microsphere arrays
AssigneeIllumina, Inc.
InventorsMark Chee, Todd Dickinson, Kevin Gunderson, Don O'Neil, John Stuelpnagel
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Abstract
The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.
Claims18
A microfluidic device for the detection of target analytes comprising:
- a) a sample inlet port;
- b) a detection inlet port;
- c) a substrate comprising:
- i) at least one sample handling well;
- ii) a first microchannel to allow fluid contact between said sample inlet port and said sample handling well;
- iii) a detection module comprising:
- (a) a plurality of discrete sites in said substrate;
- and
- (b) a population of microspheres comprising at least a first and a second subpopulation, wherein each subpopulation comprises a bioactive agent;
- wherein said microspheres are distributed on said discrete sites, wherein each discrete site contains not more than one microsphere;
- and
- iv) a second microchannel to allow fluid contact between said sample handling well and said detection inlet port.
9 more elements
A microfluidic device comprising a substrate comprising:
- a) a plurality of sample handling wells;
- b) a detection channel comprising:
- i) a plurality of discrete sites;
- and
- ii) a population of microspheres comprising at least a first and a second subpopulation, wherein each subpopulation comprises a bioactive agent, wherein said microspheres are distributed on said discrete sites;
- and
- c) at least one microchannel to allow fluid contact between each of said sample handling wells and said detection channel.
4 more elements
A method of assembling a detector in a microfluidic device comprising:
- a) providing a microfluidic device comprising:
- i) a first microchannel to allow fluid contact between a sample inlet port and a sample handling well;
- ii) a second microchannel to allow fluid contact between said sample handling well and a detection inlet port;
- and
- ii) a detection module comprising a substrate with a surface comprising discrete sites;
- b) flowing a fluid across said substrate, said fluid comprising a population of microspheres comprising at least a first and a second subpopulation, wherein each subpopulation comprises a bioactive agent, whereby said beads flow across said discrete sites, and are deposited randomly in said discrete sites;
- and
- c) reversing the flow of said fluid.
5 more elements
A method of assembling a detector in a microfluidic device comprising:
- a) providing a microfluidic device comprising:
- i) a plurality of first micro channels;
- ii) a population of microspheres in each of said microchannels;
- and
- iii) at least one receiving chamber connected to said microchannels.
- b) flowing said microspheres through said microchannels into said receiving chamber.
3 more elements
Dependencies are inferred from the claim text.