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US 2007/0184454 A1Published application
Methods and compositons for enhancing discrimination between perfect match and mismatch hybridization
AssigneeAffymetrix, Inc.
InventorKeith Jones
Life of this patent
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Abstract
Methods and compositions are provided for enhancing discrimination between perfect match and mismatch hybridization. The methods and compositions are particularly useful for genotyping analyses, gene expression analyses and diagnostic applications.
Claims15
A method of nucleic acid analysis comprising:
- hybridizing a perfect match oligonucleotide probe with a target nucleic acid, wherein the perfect match oligonucleotide probe has a nucleotide analog in the interrogating position;
- hybridizing a mismatch oligonucleotide probe with the target nucleic acid, wherein the mismatch oligonucleotide probe has a nucleotide analog in the interrogating position;
- and
- comparing perfect match hybridization intensity and mismatch hybridization intensity.
1 more element
A collection of oligonucleotide probes comprising:
- at least one prefect match oligonucleotide probe, wherein the perfect match oligonucleotide probe has a nucleotide analog in the interrogating position;
- and at least one mismatch oligonucleotide probe, wherein the mismatch oligonucleotide probe has a nucleotide analog in the interrogating position.
Dependencies are inferred from the claim text.