Agents for and method of quantifying binding
AssigneeBadrilla Limited
InventorJohn Colyer
Life of this patent
Estimating the term…
Abstract
The present invention provides a presentation system and method of use for quantifying a target moiety in a sample which may contain the target moiety, the method comprising using a specified concentration or varying the concentration of a presentation system in order to generate a comparison point or calibration curve which provides means for comparing a signal generated by the presentation system and a signal generated by a sample, wherein said presentation system comprises at least one copy of said target moiety or part thereof.
Claims32
Use of a non-natural presentation system in quantifying an amount of a target moiety which is present in a sample, the presentation system comprising at least one copy of a target moiety or part thereof that is recognizable by a binding partner and at least one domain of a scaffold material covalently linked to said target moiety wherein the scaffold material has a controllable property, and wherein the at least one domain of the scaffold is non-reactive to a binding partner specific to said target moiety or part thereof.
2-27. (canceled)
A product for quantifying the amount of a target moiety which may be present in a sample, the product comprising a plurality of presentation systems, each presentation system comprising at least one copy of a target moiety or part thereof and at least one domain covalently linked to said copy of the target moiety, wherein the domain(s) is/are non-reactive to a binding partner specific to said copy of the target moiety or part thereof, further wherein each presentation system has a different molecular weight from other presentation systems in the product.
(canceled)
A kit for quantifying the amount of a target moiety in a sample, the kit comprising a presentation system of claim 57.
A method of quantifying the amount of target moiety in a sample, the method comprising:
- a) providing a presentation system comprising at least one copy of the target moiety or part thereof that is recognizable by a binding partner and at least one domain which is non-reactive to said binding partner, said at least one copy of the target moiety being covaTently bonded to the at least one domain of a scaffold material that has a controllable property;
- b) carrying out a separation detection technique on said presentation system, wherein said presentation system is present in a specific amount;
- c) generating at least one comparison point comprising an intensity of a signal produced by the presentation system versus the amount of the presentation system.
(canceled)
A method for quantifying an amount of a protein epitope in a sample, said method comprising:
- (a) providing a protein presentation system comprising at least one copy of the protein epitope and at least one further protein domain, wherein said presentation system is of known molecular weight;
- b) carrying out a Western blot experiment on said presentation system, wherein said presentation system is in a specific concentration;
- wherein said Western blot experiment utilizes a binding partner specific to the target moiety;
- and further wherein said protein domain of the presentation system is non-reactive to the binding partner;
- and
- c) generating a comparison point comprising an intensity of a signal produced by the presentation system in said technique compared to the concentration of the presentation system.
(canceled)
A product comprising at least one copy of a target moiety or part thereof that is recognizable by a binding partner and at least one domain of a scaffold material covalently linked to said target moiety wherein the scaffold material has a controllable property, and wherein the at least one domain of the scaffold is non-reactive to a binding partner specific to said target moiety or part thereof, the target moiety being selected from the group comprising A1, PS-38 or PT17 peptides and the scaffold material being selected from the group comprising:
- an I27 domain from titin;
- I39 domain which is a subunit (subunit 5) of splicing factor 3b;
- organ of Corti protein (Mus musculus);
- heat shock protein, mitochondrial (Mus musculus);
- splicing factor 3B subunit 5 (Mus muscLilus);
- ubiquinol-cytochrome C reductase complex ubiquinone-binding protein;
- E1B protein (Human adenovirus type 11);
- chaperonin (Arabidopsis thaliana);
- photosystem II reaction center H protein (Arabidopsis thaliana);
- a NADH-ubiquinone oxidoreductase subunit, mitochondrial [Precursor] (Homo sapiens);
- signal recognition particle protein (Mus musculus);
- and DNA polymerase delta subunit 4(Mus musculus).
A non-natural presentation system, comprising at least one copy of a target moiety or part thereof that is recognizable by a binding partner and at least one domain of a scaffold material covalently linked to said target moiety wherein the scaffold material has a controllable property, and wherein the at least one domain of the scaffold is non-reactive to a binding partner specific to said target moiety or part thereof.
Dependencies are inferred from the claim text.