Streptococcus pneumoniae knockout mutants
AssigneeChiron Corporation
InventorAntonello Covacci
Life of this patent
Estimating the term…
Abstract
91 genes have been identified in Streptococcus pneumoniae that, when knocked out, result in a lethal phenotype. A further 10 genes have been identified that, when knocked out, result in poor growth characteristics when cultured in the absence of blood. These 101 genes are essential to bacterial growth and are thus useful antibiotic targets. Their invention includes knockout mutants for these 101 genes and screening methods involving the protein products of the 101 genes.
Claims10
A Streptococcus Pneumoniae bacterium in which expression of one or more of the following genes has been knocked out:
- SP0005, SP0032, SP0047, SP0056, SP0092, SP0102, SP0103, SP0253, SP0261, SP0289, SP0290, SP0292, SP0336, SP0337,SP0381, SP0382, SP0383, SP0397, SP0402, SP0417, SP0418, SP0419, SP0420,SP0423, SP0424, SP0425, SP0477, SP0516, SP0529, SP0605, SP0655, SP0656, SP0669, SP0680, SP0689, SP0708, SP0756, SP0757, SP0762, SP0806, SP0839, SP0865, SP0876, SP0935, SP0944, SP0945, SP0969, SP0974, SP0988, SP1067, SP1079, SP1084, SP1117, SP1128, SP1161, SP1263, SP1267, SP1268, SP1269, SP1271, SP1272, SP1273, SP1329, SP1360, SP1366, SP1367, SP1390, SP1420, SP1456, SP1458, SP1492, SP1521, SP1529, SP1530, SP1534, SP1559, SP1571, SP1589, SP1610, SP1649, SP1650, SP1655, SP1667, SP1670, SP1690, SP1698, SP1699, SP1709, SP1726, SP1735, SP1814, SP1881, SP1906, SP1907, SP1968, SP1975, SP2012, SP2047, SP2051, SP2146, and/or SP2216, wherein the SPnnnn nomenclature refers to the gene numbering assigned to the S. pneumoniae TIGR4 strain in Tettelin et al. (2001) Science 293:498-506.
A process for determining whether a test compound binds to a target polypeptide, comprising the steps of:
- (a) contacting the test compound with the target polypeptide to form a mixture;
- (b) incubating the mixture to allow the compound and the target polypeptide to interact;
- and (c) determining whether the compound and polypeptide interact, wherein the target polypeptide is selected from the group consisting of SP0005, SP0032, SP0047, SP0056, SP0092, SP0102, SP0103, SP0253, SP0261, SP0289, SP0290, SP0292, SP0336, SP0337, SP0381, SP0382, SP0383, SP0397, SP0402, SP0417, SP0418, SP0419, SP0420, SP0423, SP0424, SP0425, SP0477, SP0516, SP0529, SP0605, SP0655, SP0656, SP0669, SP0680, SP0689, SP0708, SP0756, SP0757, SP0762, SP0806, SP0839, SP0865, SP0876, SP0935, SP0944, SP0945, SP0969, SP0974, SP0988, SP1067, SP1079, SP1084, SP1117, SP1128, SP1161, SP1263, SP1267, SP1268, SP1269, SP1271, SP1272, SP1273, SP1329, SP1360, SP1366, SP1367, SP1390, SP1420, SP1456, SP1458, SP1492, SP1521, SP1529, SP1530, SP1534, SP1559, SP1571, SP1589, SP1610, SP1649, SP1650, SP1655, SP1667, SP1670, SP1690, SP1698, SP1699, SP1709, SP1726, SP1735, SP1814, SP1881, SP1906, SP1907, SP1968, SP1975, SP2012, SP2047, SP2051, SP2146, and/or SP2216, wherein the SPnnnn nomenclature refers to the gene numbering assigned to the S. pneumoniae TIGR4 strain in Tettelin et al. (2001) Science 293:498-506.
Dependencies are inferred from the claim text.