Back to results
US 2007/0184440 A1Published application
Methods of screening for useful proteins
OwnerNaoto Nemoto, Jun-ichi Yamaguchiinventors
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
OwnerNaoto Nemoto, Jun-ichi Yamaguchiinventors
Estimating the term…
Objectives of the present invention are achieved through, for example, methods for identifying useful proteins by synthesizing a protein that randomly comprises a disulfide bond/disulfide bonds, via random introduction of cysteine residues into the amino acid sequence, and analyzing the function of the protein, wherein the method comprises the steps of: (a) preparing one or more mRNAs encoding a protein that comprise at least two cysteine residues, and linking each of the prepared mRNAs with puromycin or a puromycin-like compound to obtain a mRNA-puromycin conjugate(s); (b) contacting a translation system with the mRNA-puromycin conjugate(s) obtained in step (a) to synthesize the protein(s), and preparing a mRNA-puromycin-protein conjugate(s); and (c) contacting one or more target substances with the mRNA-puromycin-protein conjugate(s) prepared in step (b), and determining whether the target substance(s) interacts/interact with any one of the proteins of the mRNA-puromycin-protein conjugate(s).
A method of screening for useful proteins by synthesizing a protein comprising a disulfide bond via the introduction of cysteine residues into the amino acid sequence of the protein, and analyzing the function of the protein, wherein the method comprises the steps of:
A method of screening for useful proteins by synthesizing a protein comprising a disulfide bond via the introduction of cysteine residues into the amino acid sequence of the protein, and analyzing the function of the protein, wherein the method comprises the steps of:
A synthetic protein which has 8 to 500 amino acid residues, comprises 2 to 10 cysteine residues for forming disulfide bonds, and has an association constant to the target substance that changes due to oxidation and reduction.
Dependencies are inferred from the claim text.