Method for the detection of legionella-type bacteria
AssigneeScanbec GmbH
InventorsAntje Breitenstein, Peter Neubauer, Tarja Rosilainen
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Abstract
The invention concerns a method for the detection of bacteria of the genus Legionella using a sandwich hybridization procedure at temperatures between 50 and 55° C. According to the invention new oligonucleotide probes are utilized as capture and detection probes enabling a genus or species specific detection of different Legionella bacteria. Advantageous is the exchangeability of capture and detection probes against each other for genus or species specific detections of Legionella bacteria. Furthermore the detection procedure can be performed with combinations of oligonucleotide probes for a genus and species specific detection.
Claims3
Method for the detection of bacteria of the genus Legionella by means of a sandwich hybridization procedure characterized as follows:
- a) For the genus specific detection of Legionella an oligonucleotide probe of the sequence 5′-CCTCCTCCCCACTGAAAGT-3′ is used as detection probe and an oligonucleotide probe of the sequence 5′-CACTGTATGTCAAGGGTAGG-3′ is used as capture probe.
- b) For the species specific detection of Legionella pneumophila an oligonucleotide probe of the sequence 5′-ATCTGACCGTCCCAGGTT-3′ is used as capture probe and an oligonucleotide probe of the sequence 5′-TTCGCCGCCCTCTGTATCG-3′ is used as detection probe.
- c) For the species specific detection of Legionella feelei an oligonucleotide probe of the sequence 5′-GCGCCACTAACCTCATTAT-3′ is used as capture probe and an oligonucleotide probe of the sequence 5′-TATACAACCACCTACGCACC-3′ is used as detection probe.
- d) For the species specific detection of Legionella jordanis an oligonucleotide probe of the sequence 5′-CCACTCCTCCCCACTGAAAG-3′ is used as capture probe and an oligonucleotide probe of the sequence 5′-CTTACGGTCCCCAGCTTTT-3′ is used as detection probe.
- All hybridizations are performed at temperatures between 50 and 55° C.
Dependencies are inferred from the claim text.