Compositions for use in identification of influenza viruses
OwnerRangarajan Sampath, Thomas Hall +2inventors
InventorsRangarajan Sampath, Thomas Hall, Mark Eshoo, Feng Li
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Abstract
The present invention provides oligonucleotide primers, compositions, and kits containing the same for rapid identification of viruses which are members of the influenza virus family by amplification of a segment of viral nucleic acid followed by molecular mass analysis.
Claims40
A composition comprising a purified oligonucleotide primer pair wherein each member of said primer pair is 20 to 35 nucleobases in length and wherein the forward primer comprises at least 70% sequence identity with SEQ ID NO:
- 39 and the reverse primer comprises at least 70% sequence identity with SEQ ID NO:
- 40.
A kit comprising an oligonucleotide primer pair wherein each primer of said primer pair is 20 to 35 nucleobases in length and wherein the forward primer comprises at least 70% sequence identity with SEQ ID NO:
- 39 and the reverse primer comprises at least 70% sequence identity with SEQ ID NO:
- 40.
-59. (canceled)
A method for identifying an influenza virus comprising:
- a) obtaining a sample suspected of comprising at least one bioagent;
- b) amplifying one or more nucleic acids from said sample using a primer pair configured to generate amplicons from two or more members of the orthomyxoviridae family by hybridizing a forward primer and a reverse primer to conserved regions of a M1 encoding gene, said conserved regions flanking a region that varies between said two or more members of said orthomyxoviridae family wherein said amplifying of said one or more nucleic acids results in at least one amplicon and wherein said at least one amplicon is between 45 consecutive nucleobases in length and 200 consecutive nucleobases in length;
- and
- c) determining a molecular mass of said at least one amplicon using a mass spectrometer.
A composition comprising a primer pair configured to generate amplicons from two or more members of the orthomyxoviridae family by hybridizing a forward primer and a reverse primer to conserved regions of a M1 encoding gene in two or more members of said orthomyxoviridae family, said primer pair comprising a forward primer of at least 15 oligonucleotides and reverse primer of at least 15 oligonucleotides, said conserved regions flanking a region that varies between said two or more members of said orthomyxoviridae family wherein upon amplification of a nucleic acid from a member of said orthomyxoviridae family said primer pair generates an amplicon between 45 consecutive nucleobases in length and 200 consecutive nucleobases in length.
Dependencies are inferred from the claim text.