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US 2007/0184433 A1Published application
Microparticle based biochip systems and uses thereof
OwnerShengce Tao, Guoqing Wang +1inventors
InventorsShengce Tao, Guoqing Wang, Jing Cheng
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Abstract
This invention relates generally to the field of analyte assays. In particular, the invention provides a device for analyzing an analyte, which device comprises, inter alia, various means for moving analytes and other items to facilitate binding between analytes and their binding reagents immobilized on a surface and to facilitate clearance of undesirable items away from analyte-binding reagent interaction area to reduce background noise in the assay. Methods for analyzing an analyte using the devices are also disclosed.
Claims46
A device for analyzing an analyte, which device comprises:
- a) a controllably closed space enclosed by a suitable material on a substrate, wherein said suitable material is thermoconductive, biocompatible and does not inhibit binding between an analyte and a reactant, and said controllably closed space comprising, on the surface of said substrate, a first immobilized reactant capable of binding to said analyte;
- b) a first means for controllably moving said analyte to said first immobilized reactant;
- c) a second means for controllably moving said analyte to a labeled unimmobilized complex comprising a second reactant capable of binding to said analyte and a microparticle;
- and
- d) a third means for controllably moving said labeled unimmobilized complex unbound to said analyte away from said first immobilized reactant,
- and wherein addition of a sample comprising said analyte and said labeled unimmobilized complex into said controllably closed space and operation of said means result in formation of a sandwich of said labeled unimmobilized complex-said analyte-said first immobilized reactant on said substrate.
3 more elements
Dependencies are inferred from the claim text.