Fibronectin-modified ecm tissue graft constructs and methods for preparation and use thereof
OwnerChad Johnson, Edith Nihsen +2inventors
InventorsChad Johnson, Edith Nihsen, David Zopf, Abram Janis
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Abstract
Described are modified submucosa and other extracellular matrix materials incorporating an amount of bound, exogenous fibronectin. Further described are such materials also having an amount of exogenous heparin bound to the exogenous fibronectin, and also potentially an amount of an exogenous bioactive material, such as a growth factor, bound to the exogenous heparin. Such materials may be used in methods for the treatment of wounds in patients.
Claims33
A medical graft material, comprising:
- submucosa;
- and
- an extracellular matrix material (ECM) comprising exogenous fibronectin;
- and
- wherein said exogenous fibronectin is bound to said ECM;
- and
- wherein said submucosa is lyophilized.
A method for preparing a medical graft material, comprising:
- first contacting an extracellular matrix material (ECM) comprising submucosa with a liquid medium containing fibronectin so as to prepare a modified ECM material incorporating fibronectin that is bound to the ECM and fibronectin that is not bound to the ECM;
- and
- rinsing the modified ECM to remove at least a portion of the fibronectin that is not bound to the ECM.
A medical graft material, comprising:
- a remodelable collagenous extracellular matrix material (ECM);
- and
- exogenous fibronectin molecules incorporated in or on the ECM, wherein at least 50% of the exogenous fibronectin incorporated in or on the ECM is bound to the ECM.
A method for preparing a modified extracellular matrix material, comprising:
- providing an extracellular matrix material;
- contacting the extracellular matrix material with an amount of exogenous fibronectin so as to prepare a first modified extracellular matrix material having fibronectin molecules bound to the extracellular matrix material;
- rinsing the modified extracellular matrix material to remove at least a portion of the fibronectin that is not bound to the material;
- and
- contacting the first modified extracellular matrix material with an amount of exogenous heparin and/or heparin sulfate so as to prepare a second modified extracellular matrix material having exogenous heparin and/or heparin sulfate molecules bound to the exogenous fibronectin bound to the extracellular matrix material.
A modified collagenous matrix material, comprising:
- a collagenous matrix scaffold;
- fibronectin molecules incorporated in or on the collagenous matrix scaffold wherein at least 50% of the exogenous fibronectin incorporated in or on the scaffold is bound to the scaffold;
- and
- heparin and/or heparin sulfate molecules bound to said fibronectin molecules.
Dependencies are inferred from the claim text.